LOCUS       40924_43243        6242 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pTha-M1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Tanaka R, Kikutani S, Mahardika A, Matsuda Y.
  TITLE     Localization of enzymes relating to C4 organic acid metabolisms in 
            the marine diatom, Thalassiosira pseudonana
  JOURNAL   Photosyn. Res. 121 (2-3), 251-263 (2014)
  PUBMED    24414292
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Kikutani S, Matsuda Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin 
            University, Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo 
            669-1337, Japan
REFERENCE   3  (bases 1 to 6242)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosyn. 
            Res. 121 (2-3), 251-263 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin University, 
            Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             701..1417
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      1429..1931
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="terminator"
     regulatory      1958..2916
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="promoter"
     CDS             2924..3490
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      3493..3996
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="terminator"
     primer_bind     complement(3997..4013)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4054..4072)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4093..4109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4117..4133)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4141..4171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4186..4207)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4495..5083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5257..6114)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6115..6219)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc atggtgagca agggcgagga
      721 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      781 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      841 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      901 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      961 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1021 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1081 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1141 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1201 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1261 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1321 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1381 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcat actggattgg
     1441 tgaatcaatg agccgtagca caatggttac attcggctag ctaagatcca atggcaagga
     1501 ccaagtgctg gaacttgttt tgctttagca gatcttagcg tgagaggtat ttgtcctctg
     1561 tcaggagtag atagtagatg ttctttttaa actaaaatgc taactgttcc gaattcctca
     1621 tcgcagctaa tccgtacatc aaaagacaaa atgctaggta tgtgtactac atctcctgtt
     1681 gctagataag acatatgata ggaaacacac catcaatagt cattgtagct ttacttatac
     1741 tacgcatttg cactttcccc tgagtggcag aggcgcattg agaaaatcga tctcaacata
     1801 gtttatgtag catcccctag atccattacg ttaagtctcc ttcgtctttg gtgtaggcat
     1861 gttggacaca acgaggtaaa acacaacaca aacaatgtgt ccagcaaagt agtagctgct
     1921 ccagttctcc catcgaattc ctgcagcccg ggggatctcc gagaactccc cataagtcaa
     1981 cggctccaat caagaatgta tccgacaacg gcgagcatag caacacgtcc gtctttggag
     2041 tagaatcatc atgttgtgga tgaatacaca gatgaatgac attaaaagca tgaacatgtt
     2101 agagagtagg aggtagagat tgatatggta gcattgcgat gtttgttttt ggtcagcata
     2161 tgatgagtgg ataccaatat gatgaaagtt gaatctcgcg tttgagctca gcggtacgtt
     2221 attgatcgaa agtagcctga tcaaaatcct tggagagtac aagaggatca aagaatccag
     2281 tgggggcgat aactccaagc tcgttctcaa agaggcaatg gaggtagaaa ctcatcccag
     2341 ttgagaagaa gtgaaggcag tggcggtggc gaaagcagag gcaacgagga cagacttcct
     2401 gtgggttgat gcaacgaata tttccagaag gagaagttta gagagttgaa ccgctaccta
     2461 caatgacaaa gtatcgtatc gattttgatg ttggttggtt atgaattcaa actgtaagtt
     2521 ggattgtgag aagatcagaa gttgaacgaa cacatctttc cgatcattca cctccacact
     2581 gcaacaacac ggtacttctt ccgcggcagg tctctgtcgc cattctcttg tcctgttgtt
     2641 ggctgtgaga cgaggaaagc aacgacaagt ttcacaaaag ggagttcctt taacgagata
     2701 tgttttttta taaagagtcc caatagaaag acaaattgat tcctccgtgc aaacgcgcaa
     2761 ataaacacca cgtccattat atccatatct ttcagagtat ccaacaagtg ttgaaggaca
     2821 ggtagttgaa gtaacgtatc ttccccctcg actggatcca tcaacaaggc gaacaaatcc
     2881 attcaacctc tcataaatta tctgatttac caaaccgatc caaatgacca ctcttgacga
     2941 cacggcttac cggtaccgca ccagtgtccc gggggacgcc gaggccatcg aggcactgga
     3001 tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg gcttcaccct
     3061 gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg aatcggacga
     3121 cgaatcggac gacggggagg acggcgaccc ggactcccgg acgttcgtcg cgtacgggga
     3181 cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc gccggctgac
     3241 cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc gcgcgttgat
     3301 ggggctcgcg acggagttcg cccgcgagcg gggcgccggg cacctctggc tggaggtcac
     3361 caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc tctgcggcct
     3421 ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct acatgagcat
     3481 gccctgcccc tgaatactgg attggtgaat caatgagccg tagcacaatg gttacattcg
     3541 gctagctaag atccaatggc aaggaccaag tgctggaact tgttttgctt tagcagatct
     3601 tagcgtgaga ggtatttgtc ctctgtcagg agtagatagt agatgttctt tttaaactaa
     3661 aatgctaact gttccgaatt cctcatcgca gctaatccgt acatcaaaag acaaaatgct
     3721 aggtatgtgt actacatctc ctgttgctag ataagacata tgataggaaa cacaccatca
     3781 atagtcattg tagctttact tatactacgc atttgcactt tcccctgagt ggcagaggcg
     3841 cattgagaaa atcgatctca acatagttta tgtagcatcc cctagatcca ttacgttaag
     3901 tctccttcgt ctttggtgta ggcatgttgg acacaacgag gtaaaacaca acacaaacaa
     3961 tgtgtccagc aaagtagtag ctgctccagt tctcccgatc cactagttct agagcggccg
     4021 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     4081 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4141 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4201 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4261 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6241 ac
//
